Analysis Note

ControlLAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.

Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho LAT (Tyr200) in CD3 treated Jurkat cell lysates. However, a decrease in signal was observed against phospho LAT (Tyr200) in untreated Jurkat cell lysates.

Detect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

LAT (Linker for activation of T cells) is a transmembrane adaptor protein that plays an essential role in T cell receptor (TCR) signal transduction pathways. LAT is phosphorylated on multiple tyrosine residues upon TCR engagement, recruits several important signaling proteins to the membrane, and functions as a scaffold for the assembly of numerous signaling complexes.

Immunogen

Epitope: Cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1

KLH-conjugated linear peptide corresponding to the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of human LAT phosphorylated at Tyrosine 200.

Linkage

Replaces: 04-467

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho LAT (Tyr200) immunoprecipitated from CD treated Jurkat cell lysates. A decrease in signal was observed against phospho LAT (Tyr200) immunoprecipitated from untreated Jurkat cell lysates.

Specificity

This antibody recognizes the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of LAT phosphorylated at Tyrosine 200.

Other homologies: Rat, Mouse, and Bovine (89% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~36 kDa observed. Uncharacterized bands may be observed in some cell lysates.